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Updated: Jan 28, 2026

Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
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Conditional Mutagenesis in Oligodendrocyte Lineage Cells

Sandra Goebbels1, Klaus-Armin Nave2

  • 1Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, Germany. sgoebbels@em.mpg.de.

Methods in Molecular Biology (Clifton, N.J.)
|March 2, 2019
PubMed

Insights

The Cre/loxP system enables precise genetic manipulation in specific brain cells, crucial for studying myelin-producing glial cells. Researchers can now easily analyze oligodendrocytes and their precursors in living mice using available tools.

Area of Science:

  • Neuroscience
  • Genetics
  • Cell Biology

Background:

  • The Cre/loxP system is vital for cell-type-specific gene targeting in neuroscience research.
  • Studying late-born glial cells, particularly oligodendrocytes, requires precise genetic tools.

Purpose of the Study:

  • To summarize strategies for targeting myelinating glial cells in mice.
  • To provide an overview of essential Cre driver lines for oligodendrocyte research.

Main Methods:

  • Utilizing the Cre/loxP system for conditional mutagenesis and imaging.
  • Employing a variety of Cre-expressing mouse lines for in vivo analyses.

Main Results:

  • A wide range of conditional mutants and Cre lines are available for oligodendrocyte studies.
  • The Cre/loxP system facilitates in vivo analysis of oligodendrocytes and their precursor cells.

Conclusions:

  • The Cre/loxP system is indispensable for advancing the study of myelinating glial cells.
  • Available Cre driver lines empower researchers to investigate oligodendrocyte function and development effectively.
Keywords:
Cell-type-specific targetingCreMyelinOligodendrocyteTransgenic mice

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