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DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...
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Related Experiment Video

Updated: Jan 28, 2026

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
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Intravital multiphoton microscopic imaging platform for ocular surface imaging.

Yueh-Feng Wu1, Chia-Yi Wang2, Tsung-Lin Yang3

  • 1Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.

Experimental Eye Research
|March 2, 2019
PubMed
Summary
This summary is machine-generated.

This study presents a novel intravital multiphoton microscopy platform for long-term, noninvasive ocular imaging in mice. The system achieves subcellular resolution, enabling detailed 4D visualization of eye structures and cell dynamics.

Keywords:
CorneaEndotheliumKeratocytesLimbal epithelial cellsNerveVessel

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Area of Science:

  • Ophthalmology
  • Microscopy
  • Cell Biology

Background:

  • Ocular imaging requires high resolution to study cellular structures.
  • Existing methods may be invasive or lack long-term monitoring capabilities.
  • Transgenic fluorescent protein (FP) expression offers potential for in vivo visualization.

Purpose of the Study:

  • To develop an intravital, noninvasive multiphoton microscopy platform for long-term ocular imaging.
  • To achieve subcellular resolution for visualizing distinct ocular structures in vivo.
  • To enable 4-dimensional (4D) ophthalmic studies using this platform.

Main Methods:

  • Utilized a multiphoton microscopy system with tunable laser output.
  • Designed a specialized mouse holder with a stereotaxic motorized stage for precise 3D imaging.
  • Employed three transgenic mouse lines with distinct fluorescent protein expressions (EGFP, R26R-GR, mT/mG) for cellular and structural visualization.
  • Collected second harmonic generation (SHG) signals for stromal architecture contrast.

Main Results:

  • Successfully obtained 3D images of the entire cornea (epithelium to endothelium) and conjunctiva with subcellular resolution in vivo.
  • Visualized specific cell types (corneal/limbal epithelial cells) and all cell nuclei using different FP expressions.
  • Outlined cell boundaries, nerve fibers, and capillaries using membrane-localized FP.
  • Monitored mitotic activity of corneal and limbal epithelial cells through time-lapsed recordings.

Conclusions:

  • Developed and validated an intravital multiphoton microscopic stereotaxic imaging platform for ophthalmic research.
  • Demonstrated the platform's capability for long-term, 4D, subcellular resolution in vivo ocular imaging.
  • This technology facilitates advanced studies of ocular surface dynamics and cellular processes.