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Updated: Jan 28, 2026

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
Published on: September 25, 2021
Effects of two fast-setting pulp-capping materials on cell viability and osteogenic differentiation in human dental
1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Conservative Dentistry and Endodontics, West China School and Hospital of Stomatology, Sichuan University, Chengdu, 610041, China; Department of Conservative Dentistry and Endodontics, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China.
Objective:
The purpose of this study was to compare the effects of two fast-setting pulp-capping materials, Biodentine (BD) and iRoot Fast Set (FS) root repair material, on the attachment, viability, migration, and differentiation of human dental pulp stem cells (hDPSCs).
Methods:
A comparative study was conducted between BD and FS material disks. Scanning electron microscope (SEM) images were used to observe the attachment of hDPSCs on the disks. A live/dead assay was used to assess the cell viability. Transwell assay was performed to study cell migration. Cell differentiation was determined by quantitative real-time polymerase chain reaction (qRT-PCR) for the analysis of osteogenic differentiation gene expression: alkaline phosphatase (ALP), collagen type I (COL1) and osteocalcin (OCN).
Results:
SEM images indicated that hDPSCs showed a well-spreading morphology on both BD and FS disks. FS significantly increased the proliferation and migration of hDPSCs on day 7 (P<0.05). Neither BD nor FS promoted the expression of osteogenic genes during the observation period.
Conclusions:
BD and FS both were beneficial to hDPSC attachment, and they had similar effects on cell osteogenic differentiation, whereas FS performed better than BD on hDPSCs proliferation and migration.
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