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Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
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Increased miR-214 expression suppresses cell migration and proliferation in Hirschsprung disease by interacting with

Liang Wu1, Wenzheng Yuan1,2, Jinhuang Chen1

  • 1Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Pediatric Research
|March 2, 2019
PubMed
Abstract

Insights

MicroRNA-214 (miR-214) is upregulated in Hirschsprung disease (HSCR) and inhibits cell proliferation and migration by downregulating PLAGL2. This finding sheds light on HSCR pathophysiology.

Area of Science:

  • Developmental biology
  • Molecular genetics
  • Gastroenterology

Background:

  • MicroRNA-214 (miR-214) is implicated in various diseases.
  • Its role in Hirschsprung disease (HSCR) pathophysiology remains largely unknown.

Purpose of the Study:

  • To investigate the biological role of miR-214 in HSCR.
  • To elucidate the molecular mechanisms underlying miR-214's function in HSCR.

Main Methods:

  • Gene expression analysis (qRT-PCR, Western blotting) in HSCR tissues.
  • Cellular assays (CCK8, EdU, Transwell, flow cytometry) in neuroblast cell lines.
  • Dual-luciferase reporter assay to identify miR-214 targets.

Main Results:

  • miR-214 expression is significantly upregulated in HSCR tissues compared to controls.
  • miR-214 suppresses proliferation and migration in HSCR-related cell lines.
  • PLAGL2 is identified as a direct target gene of miR-214, mediating its inhibitory effects.

Conclusions:

  • miR-214 plays a crucial role in HSCR pathophysiology.
  • miR-214 inhibits cell proliferation and migration by downregulating PLAGL2.
  • Targeting miR-214 may offer therapeutic potential for HSCR.

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