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Isolation of Listeria monocytogenes from raw milk
Applied and Environmental Microbiology
|February 1, 1986
Abstract:
During a recent outbreak of listeriosis, we examined 121 raw milk samples and 14 milk socks (filters). Listeria monocytogenes was recovered from 15 (12%) of 121 milk specimens and 2 (14%) of 14 milk socks. The optimal processing method consisted of cold enriching diluted milk for 1 month with culture to selective broth, followed by plating.
Insights
During a listeriosis outbreak, Listeria monocytogenes was found in 12% of raw milk samples and 14% of milk filters. Cold enrichment for one month proved optimal for detecting this pathogen.
Area of Science:
- Food safety
- Microbiology
- Public health
Background:
- Listeriosis outbreaks pose a significant public health risk.
- Raw milk is a potential vehicle for foodborne pathogens like Listeria monocytogenes.
Purpose of the Study:
- To investigate the prevalence of Listeria monocytogenes in raw milk during an outbreak.
- To determine an effective method for isolating Listeria monocytogenes from milk samples.
Main Methods:
- Analysis of 121 raw milk samples and 14 milk sock filters.
- Isolation and identification of Listeria monocytogenes.
- Evaluation of cold enrichment techniques for pathogen recovery.
Main Results:
- Listeria monocytogenes was detected in 15 out of 121 (12%) raw milk samples.
- Listeria monocytogenes was identified in 2 out of 14 (14%) milk sock filters.
- Optimal recovery was achieved through cold enrichment of diluted milk for one month, followed by selective broth culture and plating.
Conclusions:
- Raw milk and milk filters can harbor Listeria monocytogenes during outbreaks.
- A prolonged cold enrichment period is crucial for effective Listeria monocytogenes detection in milk products.