Related Experiment Video
Updated: Jan 28, 2026

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay
Published on: September 30, 2021
Application of Enzyme-Linked Immunosorbent Assay for Detection of Staphylococcal Enterotoxins in Food
1Food Sciences Laboratory, U.S. Army Natick Research and Development Command, Natick, Massachusetts 01760.
Abstract:
The enzyme-linked immunospecific assay (ELISA) is equally as sensitive as the radioimmunoassay (RIA) for detecting staphylococcal enterotoxins (SE). The substitution of an enzyme in ELISA for I-125 in RIA results in a more stable reagent and enables quantitation spectrophotometrically or, with appropriate enzymes, semi-quantitation by visual estimation. Assay procedures identical in principle to RIA are employed with, of course, the necessity to avoid the presence of enzyme inhibitors. To date, nanogram quantities of SEA, SEB and SEC have been successfully measured in food extracts. In common with RIA, sensitivity is decreased by the presence of food materials.
Related Concept Videos
Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
Enzyme-linked Receptors
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Transducer Mechanism: Enzyme-Linked Receptors
Major types that are helpful drug targets include:
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
Enzymes
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme Kinetics
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...

