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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
Published on: January 16, 2016
Glass Wool-Hydroextraction Method for Recovery of Human Enteroviruses from Shellfish
J T Tierney1, A Fassolitis1, D VAN Donsel1
1Virology Branch, Division of Microbiology, Food and Drug Administration, Cincinnati, Ohio 45226.
Abstract:
The Glass Wool-Hydroextraction Method was developed to analyze a number of foods for the presence of contaminating human enteroviruses. This method was modified to examine a variety of shellfish, including oysters and hard- and soft-shell clams. The method consistently recovered ca. 50% of viruses inoculated into shellfish at levels of ca. 10 virus units/100 g. In a multilaboratory study, the method successfully detected all but one of the eight test viruses, and the quantitative recoveries compared favorably with the control laboratory data.
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