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A Flooded Plate Loop Count Procedure 1
1Department of Nutrition & Food Sciences, Utah State University, Logan, Utah 84322.
Abstract:
A modification of the plate loop count procedure for milk samples is described which incorporates use of a 0.5-ml diluent loop-rinse onto pre-dried agar medium in a 100-mm petri plate. The sample is distributed using a glass spreader, the plate is dried and incubated using standard procedures. Colony formation was more uniform than with the conventional method. The method did not appear to significantly change the statistical characteristics of the plate loop count on raw milk samples. A correlation coefficient of .91 was obtained between the flooded and the conventional plate loop count.
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