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First Report of Cucumber leaf spot virus in Spain
Plant Disease
|March 3, 2019
Summary
Cucumber leaf spot virus (CLSV) was identified in Spain for the first time, causing chlorotic and necrotic spots on cucumber leaves. The virus was confirmed using RT-PCR and sequencing, and its transmission by Olpidium bornovanus was demonstrated.
Area of Science:
- Plant Virology
- Molecular Biology
- Agricultural Science
Background:
- Cucumber leaf spot virus (CLSV), a species within the Aureusvirus genus, presents as isometric particles with positive-sense, single-stranded RNA.
- CLSV is known to be transmitted by the chytrid fungus Olpidium bornovanus and has been previously reported in several European and Middle Eastern countries.
Purpose of the Study:
- To identify and characterize an outbreak of cucumber leaf spot virus (CLSV) in southeastern Spain.
- To confirm the presence of CLSV in Spain using molecular techniques and investigate its transmission.
Main Methods:
- Mechanical inoculation of cucumber plants with sap from symptomatic leaves to reproduce disease symptoms.
- Development and application of specific reverse transcription-polymerase chain reaction (RT-PCR) primers (CLSVU1440 and CLSVA2160) for viral detection.
- Nucleic acid sequencing of amplified products for genetic analysis and comparison with known CLSV strains.
- Transmission studies using infected rhizosphere soil and microscopic examination for the presence of Olpidium bornovanus.
Main Results:
- Symptoms suggestive of CLSV were reproduced in mechanically inoculated cucumber plants.
- RT-PCR assays yielded amplification products of the expected size (720 bp) from symptomatic leaves, confirming CLSV presence.
- Sequence analysis showed high similarity (94.5% nucleic acid, 99.1% amino acid) to published CLSV sequences.
- Cucumber plants inoculated with infected soil developed symptoms and tested positive for CLSV, with Olpidium bornovanus detected in root tissues.
Conclusions:
- This study reports the first occurrence of Cucumber leaf spot virus (CLSV) in Spain.
- The findings confirm the role of Olpidium bornovanus in CLSV transmission in the affected region.
- The molecular characterization provides valuable data for future diagnostics and management strategies against CLSV in Spain.
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