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Related Concept Videos

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Chirality02:25

Chirality

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Chirality is a term that describes the lack of mirror symmetry in an object. In other words, chiral objects cannot be superposed on their mirror images. For example, our feet are chiral, as the mirror image of the left foot, the right foot, cannot be superposed on the left foot.
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Chiral Stationary Phases for Liquid Chromatography: Recent Developments.

Joana Teixeira1, Maria Elizabeth Tiritan2,3,4, Madalena M M Pinto5,6

  • 1Laboratório de Química Orgânica e Farmacêutica, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. jbteixeira@live.com.pt.

Molecules (Basel, Switzerland)
|March 3, 2019
PubMed
Summary

Researchers continuously develop new chiral stationary phases (CSPs) for liquid chromatography (LC) to enhance enantioseparation performance and expand applications, including for ultra-high-performance LC systems.

Keywords:
chiral selectorchiral stationary phasechromatographic supportenantioseparationliquid chromatography

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Chiral stationary phases (CSPs) have evolved since 1938 for enantioseparation in liquid chromatography (LC).
  • Development focuses on improving enantioresolution, versatility, and application range.
  • The shift towards ultra-high-performance liquid chromatography (UHPLC) influences CSP design.

Purpose of the Study:

  • To review recent advancements in chiral stationary phase (CSP) development for LC.
  • To provide an overview of emerging trends and innovations in CSP technology.

Main Methods:

  • Review of recent scientific literature on CSPs.
  • Analysis of new chiral selectors, support materials, and synthetic approaches.
  • Focus on CSPs suitable for both conventional LC and UHPLC.

Main Results:

  • Introduction of novel chiral selectors and chromatographic support materials.
  • Exploration of diverse synthetic strategies for CSP preparation.
  • Advancements in reducing particle size for enhanced chromatographic performance.

Conclusions:

  • Continuous innovation in CSPs is crucial for advancing enantioseparation in LC.
  • Emerging strategies promise improved performance and broader applicability of CSPs.
  • The field is adapting to the demands of modern ultra-high-performance LC techniques.