Related Experiment Video

Updated: Jan 28, 2026

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
12:04

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

Published on: August 14, 2012

29.5K

O6-methylguanine-DNA methyltransferase (MGMT) status in neuroendocrine tumors: a randomized phase II study (MGMT-NET)

Annie Lemelin1, Marc Barritault2, Valérie Hervieu3

  • 1Department of Medical Oncology, Edouard Herriot Hospital, Hospices Civils de Lyon, Lyon, France.

Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
|March 3, 2019
PubMed
Abstract

Insights

Tumor O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation may predict response to alkylating agents (ALKY) chemotherapy in advanced neuroendocrine tumors (NETs). This trial investigates MGMT status and compares ALKY with oxaliplatin-based regimens.

Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Clinical Trials

Background:

  • Neuroendocrine tumors (NETs) are rare but increasing in incidence.
  • Alkylating agents (temozolomide, streptozotocin) are standard chemotherapy for advanced pancreatic NETs.
  • O6-methylguanine-DNA methyltransferase (MGMT) status may predict response to alkylating agents.

Purpose of the Study:

  • To evaluate the predictive value of tumor MGMT promoter (pMGMT) methylation for objective response to alkylating agents (ALKY) in advanced NETs.
  • To assess MGMT immunohistochemistry as a predictive biomarker.
  • To compare the efficacy of ALKY versus oxaliplatin-based chemotherapy (Ox).

Main Methods:

  • A national, prospective, randomized, controlled, multicenter trial.
  • Inclusion of adult patients with advanced, well-differentiated NETs requiring chemotherapy.
  • Assessment of pMGMT methylation by pyrosequencing, with ancillary comparison to immunohistochemistry.

Main Results:

  • 104 patients will be randomized (1:1 or 2:1 based on pMGMT methylation status) to ALKY or Ox arms.
  • The study aims to determine the predictive value of pMGMT methylation for treatment response.
  • Comparative efficacy of two chemotherapy regimens will be analyzed.

Conclusions:

  • MGMT promoter methylation status is being investigated as a predictive biomarker for alkylating agent chemotherapy in advanced NETs.
  • This trial will provide insights into optimizing chemotherapy selection for NET patients based on molecular markers.
Keywords:
Alkylating agentsClinical trialNeuroendocrineO6-Methylguanine-DNA methyltransferase

More Related Videos

Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids09:43

Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids

Published on: October 14, 2019

6.9K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.0K

Related Concept Videos

Bioequivalence Experimental Study Designs: Completely Randomized and Randomized Block Designs01:20

Bioequivalence Experimental Study Designs: Completely Randomized and Randomized Block Designs

Body:Bioequivalence experimental study designs are crucial methodologies used in evaluating and comparing the bioavailability of different drug products. These designs are categorized into various types: completely randomized, randomized block, repeated measures, cross and carry-over, and Latin square designs.Completely randomized designs involve randomly allocating treatments to all subjects participating in the experiment. This allocation is achieved by assigning unique random numbers to...
245
Random Error01:04

Random Error

Random or indeterminate errors originate from various uncontrollable variables, such as variations in environmental conditions, instrument imperfections, or the inherent variability of the phenomena being measured. Usually, these errors cannot be predicted, estimated, or characterized because their direction and magnitude often vary in magnitude and direction even during consecutive measurements. As a result, they are difficult to eliminate. However, the aggregate effect of these errors can be...
9.8K
Random Variables01:09

Random Variables

A random variable is a single numerical value that indicates the outcome of a procedure. The concept of random variables is fundamental to the probability theory and was introduced by a Russian mathematician, Pafnuty Chebyshev, in the mid-nineteenth century.
Uppercase letters such as X or Y denote a random variable. Lowercase letters like x or y denote the value of a random variable. If X is a random variable, then X is written in words, and x is given as a number.
For example, let X = the...
17.8K
Randomized Experiments01:13

Randomized Experiments

The randomization process involves assigning study participants randomly to experimental or control groups based on their probability of being equally assigned. Randomization is meant to eliminate selection bias and balance known and unknown confounding factors so that the control group is similar to the treatment group as much as possible. A computer program and a random number generator can be used to assign participants to groups in a way that minimizes bias.
Simple randomization
Simple...
9.0K
Random and Systematic Errors01:20

Random and Systematic Errors

Scientists always try their best to record measurements with the utmost accuracy and precision. However, sometimes errors do occur. These errors can be random or systematic. Random errors are observed due to the inconsistency or fluctuation in the measurement process, or variations in the quantity itself that is being measured. Such errors fluctuate from being greater than or less than the true value in repeated measurements. Consider a scientist measuring the length of an earthworm using a...
14.8K
Phase Diagrams02:39

Phase Diagrams

A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
50.0K