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Cholesterol Sulfotransferase SULT2B1b Modulates Sensitivity to Death Receptor Ligand TNFα in Castration-Resistant

Renee E Vickman1, Jiang Yang1, Nadia A Lanman2

  • 1Department of Comparative Pathobiology, Purdue University, West Lafayette, Indiana.

Molecular Cancer Research : MCR
|March 3, 2019
PubMed

Insights

Cholesterol sulfotransferase SULT2B1b enhances prostate cancer cell resistance to TNF-induced apoptosis. Targeting SULT2B1b may improve therapeutic responses in advanced prostate cancer.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cellular Signaling

Background:

  • Cholesterol sulfotransferase SULT2B1b influences androgen receptor activity and cell growth.
  • The precise mechanisms of SULT2B1b's role in prostate cancer remain unclear.
  • The specific advantages conferred by SULT2B1b expression in prostate cancer are not well understood.

Purpose of the Study:

  • To elucidate the mechanism by which SULT2B1b affects prostate cancer cells.
  • To investigate the role of SULT2B1b in TNF-mediated signaling and apoptosis.
  • To determine the clinical relevance of SULT2B1b expression in human prostate cancer.

Main Methods:

  • Single-cell mRNA sequencing to compare SULT2B1b knockdown (KD) versus control KD LNCaP cells.
  • Analysis of differentially expressed genes and canonical pathways, including death receptor signaling.
  • Examination of TNFα expression, NF-κB activation, and TNF-mediated apoptosis.
  • Investigation of SULT2B1b overexpression effects and correlation with human prostate cancer patient datasets.

Main Results:

  • Over 2,000 differentially expressed genes and altered pathways were identified upon SULT2B1b KD.
  • SULT2B1b KD increased TNFα expression and activated NF-κB in a TNF-dependent manner.
  • SULT2B1b KD significantly enhanced TNF-mediated apoptosis in both sensitive and resistant prostate cancer cells.
  • SULT2B1b overexpression decreased sensitivity to TNF-induced cell death.
  • Human prostate cancer data showed an inverse correlation between SULT2B1b expression and TNF-related genes (TNF, CD40LG, FADD, NFKB1).

Conclusions:

  • SULT2B1b expression in prostate cancer cells confers resistance to TNF-mediated apoptosis.
  • SULT2B1b may provide a growth advantage to prostate cancer cells.
  • Targeting SULT2B1b could enhance therapeutic efficacy of TNF treatment in advanced prostate cancer.

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