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Related Concept Videos

Types Of Column Chromatography01:29

Types Of Column Chromatography

13.5K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
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Diffusion on Chromatography Columns01:07

Diffusion on Chromatography Columns

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In column chromatography, when an analyte is introduced as a narrow band at the top of the column, the solutes begin to separate and broaden, developing a Gaussian profile. This broadening occurs due to various factors, such as longitudinal diffusion.
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

8.9K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
8.9K
Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

3.5K
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
3.5K
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

2.3K
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
2.3K
Muscles of the Vertebral Column01:27

Muscles of the Vertebral Column

3.2K
The back muscles that lie deep into the thoracolumbar fascia are called intrinsic or true back muscles. These muscles are divided into four layers: superficial, intermediate, deep, and deepest layers.
Superficial Layer:
The superficial layer consists primarily of the splenius muscles, which include the splenius capitis and splenius cervicis. These muscles are mainly responsible for the head and cervical spine movements, including extension, rotation, and lateral bending. The splenius capitis...
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Related Experiment Video

Updated: Jan 28, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

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Spun-Column Chromatography.

Michael R Green, Joseph Sambrook

    Cold Spring Harbor Protocols
    |March 3, 2019
    PubMed
    Summary

    This protocol details using Sephadex G-50 or Bio-Gel columns for effective DNA purification. These methods ensure high-quality DNA suitable for downstream molecular biology applications.

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Biotechnology

    Background:

    • DNA purification is crucial for molecular biology techniques.
    • Existing methods may require optimization for specific applications.
    • Size exclusion chromatography offers a method for DNA sample preparation.

    Purpose of the Study:

    • To provide a detailed protocol for DNA purification using size exclusion chromatography.
    • To outline the setup and operational procedures for Sephadex G-50 and Bio-Gel columns.
    • To ensure reproducible and high-purity DNA isolation.

    Main Methods:

    • Utilizing Sephadex G-50 columns for DNA purification.
    • Employing Bio-Gel columns as an alternative for DNA purification.
    • Describing column packing, equilibration, sample loading, and elution steps.

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    Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
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    Main Results:

    • Successful purification of DNA using both Sephadex G-50 and Bio-Gel columns.
    • Demonstration of effective removal of contaminants.
    • Obtained purified DNA suitable for downstream applications.

    Conclusions:

    • Sephadex G-50 and Bio-Gel columns provide reliable methods for DNA purification.
    • The described protocol facilitates the preparation of high-quality DNA.
    • This technique is valuable for researchers in molecular biology and related fields.