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Rapid analysis of intraperitoneally administered morphine in mouse plasma and brain by microchip

Elisa Ollikainen1, Teemu Aitta-Aho2, Michaela Koburg1

  • 1Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.

Scientific Reports
|March 3, 2019
PubMed

Insights

A new microchip electrophoresis-electrochemical detection (MCE-EC) method enables rapid morphine analysis in mouse plasma and brain tissue. This technique supports drug discovery by providing crucial chemical data for animal studies.

Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Neuroscience

Background:

  • * In vitro models are insufficient for fully understanding drug effects in living organisms.
  • * Animal studies are crucial for drug discovery and development.
  • * Accurate quantification of drugs in biological matrices is essential.

Purpose of the Study:

  • * To develop and validate a microchip electrophoresis-electrochemical detection (MCE-EC) method for rapid morphine analysis.
  • * To assess the method's performance in mouse plasma and brain homogenates.
  • * To apply the method in a behavioral animal study.

Main Methods:

  • * Development and qualification of a microchip electrophoresis-electrochemical detection (MCE-EC) method.
  • * Liquid-liquid extraction (LLE) for sample preparation.
  • * Analysis of morphine in mouse plasma and brain homogenates.

Main Results:

  • * The MCE-EC method demonstrated high precision (3.7-4.5% CV in plasma) and accuracy (100-107%).
  • * The method successfully quantified morphine in mouse plasma (6.7-17 µM) and brain homogenates (80-125 nM) after administration.
  • * Brain concentrations were significantly lower than plasma concentrations, requiring preconcentration.

Conclusions:

  • * The developed MCE-EC method is a feasible and rapid tool for morphine quantification in animal studies.
  • * This technique provides valuable supplementary chemical data for behavioral and pharmacological research.
  • * Microchip-based analytical systems offer advantages for drug discovery research.

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