Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Lipids01:32

Membrane Lipids

34.2K
Lipids are an essential component of all biological membranes. The average lipid content in mammalian membranes is 50%, though it can be as low as 20% in the inner mitochondrial membrane or as high as 80% in the myelin sheath present around the nerve cells.
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
34.2K
Flow Cytometry01:23

Flow Cytometry

16.1K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
16.1K
Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

2.3K
Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
2.3K
Plasma Membrane in Bacteria and Archaea01:27

Plasma Membrane in Bacteria and Archaea

1.7K
The plasma membrane is an essential cellular structure responsible for maintaining cellular integrity and regulating the selective transport of molecules. While bacteria and archaea share the fundamental function of plasma membranes, their structural and molecular differences reflect adaptations to distinct ecological and physiological challenges.Bacterial Plasma MembranesBacterial plasma membranes are predominantly composed of phospholipids with fatty acid chains ester-linked to a glycerol...
1.7K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

18.9K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
18.9K
What are Lipids?01:38

What are Lipids?

220.0K
Overview
220.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autoantibodies Predictive of Atherosclerosis Progression and Statin Response in Juvenile-Onset SLE: A Biomarker Discovery Study.

medRxiv : the preprint server for health sciences·2026
Same author

Unveiling Endotypes in Systemic Lupus Erythematosus Through Multiomic Analysis: Insights Into Cardiovascular and Renal Complications.

Arthritis & rheumatology (Hoboken, N.J.)·2026
Same author

Clinical phenotypes, classification, and long-term outcomes of childhood-onset Sjögren's disease into adulthood: a single-centre cohort study.

The Lancet. Rheumatology·2025
Same author

Simple hyperinflammation scores predict mortality in hospitalized patients with COVID-19 and offer a personalized medicine approach to dexamethasone intervention.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2025
Same author

Subclinical Atherosclerosis Risk Can Be Predicted in Female Patients With Systemic Lupus Erythematosus Using Metabolomic Signatures: An Observational Study.

Journal of the American Heart Association·2025
Same author

Estrogen influences class-switched memory B cell frequency only in humans with two X chromosomes.

The Journal of experimental medicine·2025

Related Experiment Video

Updated: Jan 28, 2026

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
05:39

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry

Published on: November 7, 2010

27.1K

Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry.

Kirsty E Waddington1,2, Inés Pineda-Torra1, Elizabeth C Jury3

  • 1Division of Medicine, Centre for Cardiometabolic Medicine, University College of London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2019
PubMed
Summary
This summary is machine-generated.

This study presents flow cytometry methods to measure lipid rafts in human CD4+ T cells. These techniques quantify glycosphingolipids and cholesterol, aiding research into T-cell function and autoimmune diseases.

Keywords:
CholesterolFlow cytometryGlycosphingolipidsLipid raftsT cellsdi-4-ANEPPDHQ

More Related Videos

Flow Cytometry Purification of Mouse Meiotic Cells
10:43

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

18.3K
Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
05:38

Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry

Published on: January 28, 2017

12.8K

Related Experiment Videos

Last Updated: Jan 28, 2026

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
05:39

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry

Published on: November 7, 2010

27.1K
Flow Cytometry Purification of Mouse Meiotic Cells
10:43

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

18.3K
Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
05:38

Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry

Published on: January 28, 2017

12.8K

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Plasma membrane lipid rafts are crucial for T-cell antigen receptor (TCR) signaling.
  • Lipid raft composition impacts human CD4+ T-cell function.
  • Defects in lipid rafts are linked to CD4+ T-cell dysfunction in autoimmunity.

Purpose of the Study:

  • To provide quantitative flow cytometry methods for analyzing plasma membrane lipid composition in primary human CD4+ T cells.
  • To enable detailed investigation of lipid raft alterations in T-cell function and disease.

Main Methods:

  • Quantification of glycosphingolipid expression using cholera toxin subunit B.
  • Measurement of cholesterol levels via filipin staining.
  • Assessment of membrane lipid order using the fluorescent probe di-4-ANEPPDHQ.

Main Results:

  • Established three distinct flow cytometry protocols for lipid raft analysis.
  • Demonstrated the utility of these methods for primary human CD4+ T cells.

Conclusions:

  • These adaptable flow cytometry methods offer a robust approach to quantitatively assess plasma membrane lipid composition.
  • The techniques facilitate deeper understanding of lipid raft roles in T-cell signaling and immune-related disorders.