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Updated: Jan 28, 2026

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Published on: February 25, 2021
Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates
Mariana P Stelling1,2, Ananda A de Bento1,2, Philippe Caloba1,2
1Laboratório de Bioquímica e Biologia Celular de Glicoconjugados, Programa de Glicobiologia, Instituto de Bioquímica Médica Leopoldo de Meis and Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Abstract:
Marine invertebrates produce different kinds of sulfated polysaccharides. These glycans play essential roles in several biological processes and the study of these molecules is promising in a variety of fields. In the following sections, we describe the materials and methods used for the extraction, purification, and characterization of marine invertebrate-derived glycosaminoglycans.
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