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Updated: Jan 28, 2026

Detection of Protease Activity by Fluorescent Peptide Zymography
Published on: January 20, 2019
Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography
Elin Hadler-Olsen1,2,3, Jan-Olof Winberg4
1Faculty of Health Sciences, Department of Medical Biology, UiT-The Arctic University of Norway, Tromsø, Norway. elin.hadler-olsen@uit.no.
Abstract:
To explore the physiological or pathological roles of proteases, it is important to be able to detect and precisely localize them in a tissue, to differentiate between inactive and active forms, as well as to quantify and determine the nature of the enzyme that degrades a given substrate. Here we present a protocol for real-time gelatin zymography that is very useful for the detection of gelatin-degrading proteases in tissue extracts. This method uses fluorescence-labeled gelatin and therefore we also present an easy, fast, and cheap method for labeling gelatin with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF).
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