Long noncoding RNA 00460 (LINC00460) promotes glioma progression by negatively regulating miR-320a

Long noncoding RNA 00460 (LINC00460) has been reported to contribute to tumorigenesis in multiple types of human malignancies. However, the biological role and the underlying molecular mechanism of LINC00460 in glioma remain unclear. The aim of this study was to investigate the clinical value, the biological function, and the potential mechanism of LINC00460 in glioma.

The expression level of LINC00460 in glioma tissues and cell lines was examined by quantitative real-time polymerase chain reaction (qRT-PCR). Cell Counting Kit-8, flow cemetery, wound healing, and transwell invasion assays were used to explore the effect of LINC00460 on glioma cell proliferation, apoptosis, migration, and invasion. qRT-PCR and reporter assays were used to further verify the regulatory mechanism of LINC00460 in glioma progression.

LINC00460 expression was upregulated in glioma tissues and cell lines compared with non-tumor brain samples and astrocyte cell line (NHA), respectively. Moreover, increased LINC00460 expression was closely associated with glioma tumor grade. Loss-of-function assays revealed that knockdown of LINC00460 significantly inhibited glioma cell proliferation, induced cell apoptosis, and suppressed migration and invasion. The mechanistic assays disclosed that LINC00460 binded to miR-320a in a sequence-specific manner and regulated its expression. Moreover, miR-320 inhibition partially attenuated LINC00460 knockdown-mediated suppressive effects on glioma cell proliferation, migration, and invasion.

These findings suggested that LINC00460 might function as an oncogenic lncRNA in glioma development and could be explored as a potential therapeutic target for glioma.