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Proposal of Lysobacter pythonis sp. nov. isolated from royal pythons (Python regius).

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A novel bacterial species, Lysobacter pythonis, was identified from royal pythons. This discovery expands our understanding of reptile-associated bacteria and introduces a new species within the Lysobacter genus.

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Area of Science:

  • Microbiology
  • Genomics
  • Herpetology

Background:

  • Bacterial strains were isolated from the respiratory tracts of royal pythons.
  • Previous studies have characterized bacteria from reptile respiratory systems.

Purpose of the Study:

  • To taxonomically characterize two bacterial strains, 4284/11T and 812/17, isolated from royal pythons.
  • To determine if these strains represent a novel species.

Main Methods:

  • 16S rRNA gene sequencing
  • 16S-23S rRNA internal transcribed spacer (ITS) sequencing
  • Partial groEL gene sequencing
  • Genomic fingerprinting
  • In silico DNA-DNA hybridization (DDH) using ANIb and gANI
  • Core gene phylogeny
  • Chemotaxonomic analyses (polyamine, quinone, polar lipid, fatty acid profiles)
  • Physiological tests

Main Results:

  • The two strains, 4284/11T and 812/17, had identical 16S rRNA, ITS, and partial groEL gene sequences.
  • Genomic analyses (ANIb, gANI) indicated that strain 4284/11T represents a novel species, with Lysobacter tolerans as its closest relative.
  • Phylogenetic and chemotaxonomic data supported assignment to the genus Lysobacter.
  • Physiological tests differentiated the strains from closely related species.

Conclusions:

  • The novel bacterial species Lysobacter pythonis sp. nov. is proposed.
  • Strain 4284/11T is designated as the type strain (CCM 8829T).
  • Strain 812/17 is identified as a second isolate of this new species.