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Rat-derived feeder cells immortalized by expression of mutant CDK4, cyclin D, and telomerase can support stem cell

Masafumi Katayama1, Tohru Kiyono2, Kengo Kuroda3

  • 1National Institute for Environmental Studies, Center for Environmental Biology and Ecosystem Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan; National Institute for Environmental studies, Wildlife Genome Collaborative Research Group, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.

Biochimica Et Biophysica Acta. Molecular Cell Research
|March 4, 2019
PubMed

Insights

New rat-derived feeder cells support stem cell cultures for up to 14 days. These immortalized fibroblasts overcome limitations of traditional mouse embryonic fibroblasts (MEFs) and other feeder cells for extended stem cell research.

Area of Science:

  • Stem cell biology
  • Cell culture technology
  • Fibroblast research

Background:

  • Stem cell maintenance often relies on feeder cells.
  • Traditional feeder cells like mouse embryonic fibroblasts (MEFs) have limitations, including finite expansion.
  • Existing immortalized feeder cells support cultures for only 4-7 days.

Purpose of the Study:

  • To develop novel immortalized feeder cells for extended stem cell culture.
  • To overcome the limitations of traditional and existing immortalized feeder cells.

Main Methods:

  • Establishment of rat-derived fibroblasts.
  • Immortalization using mutant cyclin-dependent kinase 4, cyclin D, and telomerase reverse transcriptase.
  • Evaluation of feeder cell performance in stem cell cultures.

Main Results:

  • Newly established rat-derived fibroblasts were successfully immortalized.
  • These immortalized cells supported stem cell cultures for approximately 14 days.
  • Demonstrated extended functionality compared to previously reported immortalized feeder cells.

Conclusions:

  • The developed rat-derived immortalized fibroblasts serve as effective feeder cells.
  • These cells offer a valuable tool for prolonged stem cell culture and research.
  • Provides a more robust feeder cell solution for stem cell applications.
Keywords:
Cell cycle arrestFeeder cellImmortalized cellMitomycin CSpecies difference

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