microRNA-144 regulates the NF-κB signaling in miiuy croaker via targeting IL1β

Area of Science:

• * Molecular Biology
• * Immunology
• * Aquatic Animal Health

Background:

• * MicroRNAs (miRNAs) are key regulators of immune responses, but their precise mechanisms in fish remain unclear.
• * Understanding miRNA-mediated immune regulation is crucial for teleost fish health and aquaculture.

Purpose of the Study:

• * To investigate the regulatory role of miR-144 in the NF-κB signaling pathway in miiuy croaker.
• * To elucidate the mechanism by which miR-144 targets IL1β to modulate immune responses.

Main Methods:

• * Bioinformatic prediction of miR-144 targeting IL1β.
• * Luciferase reporter assays to validate miR-144 and IL1β interaction.
• * Overexpression and inhibition studies using miR-144 mimics and inhibitors.

Main Results:

• * miR-144 directly targets and negatively regulates IL1β expression in miiuy croaker.
• * Overexpression of miR-144 dose-dependently inhibited IL1β activity.
• * miR-144 inhibition reversed the suppression of IL1β activity.
• * miR-144 negatively regulates the nuclear factor kappaB (NF-κB) signaling pathway via IL1β targeting.

Conclusions:

• * miR-144 plays a significant role in regulating immune responses in miiuy croaker.
• * This study enhances the understanding of miRNA-mediated immune regulation in teleost fish.
• * Findings provide insights into novel immune regulatory mechanisms in fish.