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Updated: Jan 28, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Published on: January 11, 2017
Application of liposome membrane as the reaction field: A case study using the Horner-Wadsworth-Emmons reaction
Toshinori Shimanouchi1, Yuki Kitagawa1, Yukitaka Kimura1
1Graduate School of Environmental and Life Science, Okayama University, 3-1-1 Tsushima-Naka, Okayama 700-8530, Japan.
Abstract:
The properties of the liposome membrane as a reaction field were investigated by focusing on the Horner-Wadsworth-Emmons reaction as a case study. Use of the liposomes existing in the gel phase resulted in the enhanced activity of the substrates and furnished the products with same E/Z stereoselectivity as in the liposome-free system. The membrane environment in the gel phase most likely assisted the formation of adducts that induced selective generation of the E-isomer. The possible role of liposomes is to assist the proton removal from the reactant, rather than providing the basic interfacial environment.
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