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The p53 mRNA: an integral part of the cellular stress response

Lucia Haronikova1, Vanesa Olivares-Illana2, Lixiao Wang3

  • 1RECAMO, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech Republic.

Nucleic Acids Research
|March 5, 2019
PubMed

Insights

The p53 mRNA acts as a regulatory platform, controlling protein synthesis and stability. Specific mutations in this mRNA can block p53 activation, impacting cellular stress responses.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Signaling pathways converge on p53 to manage cellular stress responses, including cell cycle arrest, senescence, and apoptosis.
  • p53 activity is regulated by post-translational modifications and protein-protein interactions, optimizing genetic information for cellular diversity.

Purpose of the Study:

  • To illustrate how protein and RNA interactions involving p53 mRNA regulate RNA stability, translation efficiency, and alternative translation initiation.
  • To describe the riboswitch-like features of p53 mRNA and its control over p53 protein synthesis, stability, and nascent protein modifications.

Main Methods:

  • Review of existing literature on p53 signaling pathways, post-translational modifications, and mRNA regulation.
  • Analysis of protein-RNA interactions and their impact on p53 mRNA function.
  • Examination of riboswitch-like features within the p53 mRNA structure.

Main Results:

  • p53 mRNA interacts with proteins and signaling pathways to control RNA stability and translation.
  • The p53 mRNA functions as a platform with riboswitch-like properties, regulating p53 synthesis and protein modifications.
  • A synonymous mutation in cancer disrupts p53 mRNA folding, inhibiting p53 activation after DNA damage.

Conclusions:

  • p53 mRNA sequences have co-evolved with p53 protein function, acting as a direct target for signaling pathways.
  • The regulatory role of p53 mRNA reveals hidden information within mRNA sequences, impacting cellular responses to stress and DNA damage.

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