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Updated: Jan 28, 2026

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy
Published on: May 27, 2022
Cryogenic Ion Spectroscopy for Identification of Monosaccharide Anomers
Valeriu Scutelnic1, Thomas R Rizzo1
1Laboratory of Molecular Physical Chemistry , École Polytechnique Fédérale de Lausanne , Station 6 , CH-1015 Lausanne , Switzerland.
Abstract:
We combine conformer-selective, cryogenic infrared spectroscopy, quantum mechanical computations, and 18O substitution at the reducing end to determine the structural preferences of protonated glucosamine in the gas phase. Cryogenic infrared-infrared (IR-IR) double resonance spectroscopy of helium-tagged, protonated glucosamine provides vibrational fingerprints of individual conformers, and 18O isotopic labeling facilitates the match with computed structures and provides a selective probe of the anomeric hydroxyl. This is key for using vibrational spectroscopy for glycan analysis and determining the generality of anomeric memory during glycosidic bond cleavage.
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