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Updated: Jan 28, 2026

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
Published on: September 20, 2022
Untargeted metabolomic analysis in non-fasted diabetic dogs by UHPLC-HRMS
A L O'Kell1, T J Garrett2, C Wasserfall3
1Department of Small Animal Clinical Sciences, College of Veterinary Medicine, The University of Florida, Box 100116, 2015 SW 16th Avenue, Gainesville, FL, 32608, USA. aokell@ufl.edu.
Introduction:
We recently identified variances in serum metabolomic profiles between fasted diabetic and healthy dogs, some having similarities to those identified in human type 1 diabetes.
Objectives:
Compare untargeted metabolomic profiles in the non-fasted state.
Methods:
Serum from non-fasted diabetic (n = 6) and healthy control (n = 6) dogs were analyzed by liquid chromatography-high resolution mass spectrometry.
Results:
Clear clustering of metabolites between groups were observed, with multiple perturbations identified that were similar to those previously observed in fasted diabetic dogs.
Conclusion:
These findings further support the development of targeted assays capable of detecting metabolites that may be useful as biomarkers of canine diabetes.
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