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Updated: Jan 28, 2026

Operation of a Benchtop Bioreactor
Published on: September 12, 2013
Efficient nitrogen removal by simultaneous heterotrophic nitrifying-aerobic denitrifying bacterium in a purification
Peng Jin1, Yinyan Chen2, Tao Xu2
1The College of Agricultural and Food Sciences, Zhejiang A & F University, Hangzhou 311300, China.
Abstract:
Nitrogen removal performance of a simultaneous heterotrophic nitrifying-aerobic denitrifying (SND) bacterium (KSND) in a purification tank bioreactor (PTBR) amended with two-stage dissolved oxygen (DO) control was investigated. NH4+-N and total nitrogen (TN) removal efficiencies under aerobic conditions for domestic wastewater treatment were 97.12% and 52.64%, respectively. Under serial aerobic (DO > 4.0 mg/L) and anaerobic (DO < 0.5 mg/L) phases, average TN removal efficiency from effluent was 95.45%, without nitrate and nitrite accumulation. DO control assay demonstrated that anaerobic condition adversely affected nitrification (46.13%), but was conducive to denitrification (93.52%). Transcriptional analysis revealed 2.72-fold increase in hydroxylamine reductase expression under aerobic condition as compared to anaerobic condition. Nitrate reductase and nitric oxide reductase homologs had the additional activity of supporting anaerobic or aerobic denitrification in SND bacteria. Under two-stage DO control, KSND maintained high abundance in oligotrophic PTBR, removing 87.88% TN from low-carbon to nitrogen domestic sewage in 180-days.
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