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Transmitting Plant Viruses Using Whiteflies
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First Report of Anemone Plants Infected by Ranunculus white mottle virus.

A M Vaira1, M Vecchiati1, V Lisa1

  • 1Istituto di Fitovirologia Applicata, CNR, Torino, Italy.

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|March 6, 2019
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Summary
This summary is machine-generated.

Ranunculus white mottle virus (RWMV) was found infecting anemone crops in Italy, often in mixed infections. This marks the first report of RWMV in anemone, potentially causing severe disease symptoms.

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Area of Science:

  • Plant Pathology
  • Virology
  • Agricultural Science

Background:

  • Ranunculus white mottle virus (RWMV), an Ophiovirus, was previously identified in Ranunculus asiaticus in Italy.
  • Mixed viral infections often complicate symptom identification in ornamental crops.

Purpose of the Study:

  • To identify the causal agent of a severe disease affecting anemone (Anemone coronaria) crops in Northwest Italy.
  • To report the first occurrence of Ranunculus white mottle virus (RWMV) in anemone.

Main Methods:

  • Enzyme-linked immunosorbent assays (ELISAs) were used to detect RWMV and other common anemone viruses in field samples.
  • Electron microscopy was employed to visualize virus particles.
  • Molecular techniques, including Northern and dot blot analyses with a specific RWMV RNA2 probe, were used to confirm viral presence and multiplication.

Main Results:

  • Ranunculus white mottle virus (RWMV) was detected in seven out of 24 field samples of anemone.
  • RWMV occurred in mixed infections with Cucumber mosaic virus subgroup II or Tobacco necrosis virus.
  • Virus-like particles consistent with Ophiovirus were observed via electron microscopy.
  • Molecular tests confirmed RWMV infection and replication in inoculated test plants.

Conclusions:

  • This study reports the first detection of Ranunculus white mottle virus (RWMV) in anemone (Anemone coronaria).
  • RWMV, in mixed infections with other viruses, appears to contribute to severe disease symptoms in anemone crops.
  • Further research is needed to fully elucidate the symptomatology of RWMV in anemone.