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Updated: Jan 28, 2026

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Structural and antioxidant studies of a new arabinoxylan from green stem Andrographis paniculata (Kalmegh)
Gajendra Nath Maity1, Prasenjit Maity2, Adhiraj Dasgupta3
1Department of Microbiology, Panskura Banamali College, Panskura, Purba Midnapore, 721152, West Bengal, India.
Abstract:
A water soluble arabinoxylan (APPS), with a molecular weight ∼1.49 × 105 Da was isolated from the green stem of Andrographis paniculata. The APPS contained D-xylose, 2-methoxy D-xylose and L- arabinose in molar ratio of 3:1:1. The structure of the repeating unit in the polysaccharide was determined through several chemical (acid hydrolysis, Methylation analysis and periodate oxidation) and spectroscopic analysis (1D/2D NMR experiments) as: In vitro antioxidant assay, APPS was found to possess ferrous ion chelating activity (EC50 = 283 μg/ml), superoxide radical scavenging activity (EC50 = 470 μg/ml), and hydroxide radical scavenging activity (EC50 = 193 μg/ml). Thus, APPS could be used as a natural antioxidant agent for food and pharmaceutical industries.
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