Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetics of Speciation02:16

Genetics of Speciation

21.0K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
21.0K
What is Population Genetics?01:25

What is Population Genetics?

64.6K
A population is composed of members of the same species that simultaneously live and interact in the same area. When individuals in a population breed, they pass down their genes to their offspring. Many of these genes are polymorphic, meaning that they occur in multiple variants. Such variations of a gene are referred to as alleles. The collective set of all the alleles within a population is known as the gene pool.
64.6K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.0K
Overview
80.0K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

9.2K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.2K
Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

30.7K
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
30.7K
Mutation, Gene Flow, and Genetic Drift01:09

Mutation, Gene Flow, and Genetic Drift

63.8K
In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).
63.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visualizing C. difficile During Murine Infection.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Defining the order of assembly of the <i>Clostridioides difficile</i> divisome complex.

bioRxiv : the preprint server for biology·2026
Same author

The CspC:CspA heterodimer transduces germinant and co-germinant signals during Clostridioides difficile spore germination.

PLoS biology·2026
Same author

In situ visualization of Clostridioides difficile phenotypic heterogeneity and single-cell morphology during gut infection.

Nature communications·2026
Same author

Penicillin-binding proteins exhibit functional redundancy during asymmetric cell division in <i>Clostridioides difficile</i>.

Journal of bacteriology·2025
Same author

Molecular dissection of Class A PBP function uncovers novel features of the non-canonical Clostridioides difficile divisome complex.

PLoS genetics·2025
Same journal

Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

Journal of bacteriology·2026
Same journal

Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

Journal of bacteriology·2026
Same journal

DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

Journal of bacteriology·2026
Same journal

Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

Journal of bacteriology·2026
Same journal

Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

Journal of bacteriology·2026
Same journal

YpeB stability affects germination possibly through delaying SleB activity in <i>Bacillus subtilis</i>.

Journal of bacteriology·2026
See all related articles

Related Experiment Video

Updated: Jan 28, 2026

Development of a Larval Zebrafish Infection Model for Clostridioides difficile
09:13

Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Published on: February 14, 2020

6.8K

Expanding the Clostridioides difficile Genetics Toolbox.

Aimee Shen1

  • 1Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA. aimee.shen@tufts.edu.

Journal of Bacteriology
|March 6, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a CRISPRi system for gene silencing in Clostridioides difficile. This new tool advances C. difficile genetics, aiding the study of essential genes and physiology.

More Related Videos

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
11:13

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Published on: September 14, 2013

40.0K
Fecal Microbiota Transplantation via Colonoscopy for Recurrent C. difficile Infection
07:06

Fecal Microbiota Transplantation via Colonoscopy for Recurrent C. difficile Infection

Published on: December 8, 2014

27.7K

Related Experiment Videos

Last Updated: Jan 28, 2026

Development of a Larval Zebrafish Infection Model for Clostridioides difficile
09:13

Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Published on: February 14, 2020

6.8K
Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
11:13

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Published on: September 14, 2013

40.0K
Fecal Microbiota Transplantation via Colonoscopy for Recurrent C. difficile Infection
07:06

Fecal Microbiota Transplantation via Colonoscopy for Recurrent C. difficile Infection

Published on: December 8, 2014

27.7K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Recent advancements in Clostridioides difficile genetics have been driven by new molecular tools.
  • Existing genetic tools include allelic replacement and transposon mutagenesis.

Purpose of the Study:

  • To develop a novel CRISPR interference (CRISPRi) system for gene silencing in Clostridioides difficile.
  • To establish a tunable and robust genetic tool for C. difficile research.

Main Methods:

  • Development and implementation of a CRISPRi system for targeted gene silencing.
  • Demonstration of the system's efficacy in studying essential gene function.

Main Results:

  • The study successfully established a CRISPRi strategy for gene silencing in C. difficile.
  • The CRISPRi system demonstrated tunability and robustness.
  • The utility of the system for investigating essential gene function was highlighted.

Conclusions:

  • The developed CRISPRi system expands the genetic toolbox for Clostridioides difficile research.
  • This tool offers new possibilities for dissecting C. difficile physiology and understanding its essential genes.