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Troxerutin affects the male fertility in prepubertal type 1 diabetic male rats
Zohreh Zavvari Oskuye1, Fariba Mirzaei Bavil2, Gholam Reza Hamidian3
1Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Objectives:
Diabetes can gradually cause damage to the function and structure of male gonads. This survey was conducted to investigate the effect of troxerutin on hormonal changes, serum oxidative stress indices, and testicular function and structure in prepubertal diabetic rats.
Materials And Methods:
Fifty prepubertal (6 weeks old) male Wistar rats were divided into five groups including Control, Troxerutin, Diabetic, Diabetic+Troxerutin, and Diabetic+Insulin. Type I diabetes was induced by 55 mg/kg of streptozotocin intraperitoneally. The groups were treated with 150 mg/kg/day troxerutin via oral gavage or 4-6 IU/day insulin via subcutaneous injection for 4 consecutive weeks. Blood sugar (BS) and serum levels of insulin, FSH, LH, testosterone, glutathione peroxidase (GPX), superoxide dismutase (SOD), malondialdehyde (MDA), and total antioxidant capacity (TAC) were analyzed. Testis and epididymis were removed for histopathologic study and analysis of sperm parameters.
Results:
Troxerutin significantly reduced the BS in the diabetic group similar to insulin but could not affect insulin, FSH, or LH significantly. Troxerutin caused a significant increase in testosterone and GPX but had no significant effect on serum MDA, TAC, and SOD levels. In addition, troxerutin had a better effect than insulin on diabetes-induced testicular structural damage. Sperm analysis results also revealed that troxerutin and insulin could improve sperm number, motility, and viability in diabetic rats.
Conclusion:
According to these results, it can be derived that administration of troxerutin is a suitable protective strategy for side effects of diabetes in testis of prepubertal diabetic male rats.
Insights
Troxerutin protects prepubertal diabetic rats' testes from diabetes damage, improving testosterone and sperm quality. This natural compound offers a promising protective strategy against diabetic testicular dysfunction.
Area of Science:
- Endocrinology
- Reproductive Biology
- Pharmacology
Background:
- Diabetes mellitus can lead to significant damage to male gonadal function and structure.
- Prepubertal diabetes may have distinct long-term impacts on reproductive health.
- Oxidative stress plays a crucial role in diabetes-induced testicular damage.
Purpose of the Study:
- To investigate the protective effects of troxerutin on testicular function and structure in prepubertal diabetic rats.
- To evaluate the impact of troxerutin on hormonal profiles and serum oxidative stress indices in diabetic rats.
- To compare the efficacy of troxerutin with insulin in mitigating diabetes-induced testicular damage.
Main Methods:
- Fifty prepubertal Wistar rats were divided into Control, Troxerutin, Diabetic, Diabetic+Troxerutin, and Diabetic+Insulin groups.
- Type 1 diabetes was induced using streptozotocin; treatments included troxerutin (oral gavage) or insulin (subcutaneous injection) for 4 weeks.
- Analyses included blood glucose, serum hormones (insulin, FSH, LH, testosterone), oxidative stress markers (GPX, SOD, MDA, TAC), testicular histopathology, and sperm parameters.
Main Results:
- Troxerutin significantly reduced blood glucose levels in diabetic rats, comparable to insulin.
- Troxerutin increased serum testosterone and glutathione peroxidase (GPX) levels, with no significant effect on malondialdehyde (MDA), total antioxidant capacity (TAC), or superoxide dismutase (SOD).
- Troxerutin demonstrated superior protection against testicular structural damage compared to insulin and improved sperm number, motility, and viability in diabetic rats.
Conclusions:
- Troxerutin administration serves as an effective protective strategy against testicular side effects of diabetes in prepubertal male rats.
- Troxerutin shows potential in preserving testicular structure and function in the context of chemically induced diabetes.
- Further research into troxerutin as a therapeutic agent for diabetic reproductive complications is warranted.
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