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Updated: Jan 28, 2026

An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice
Published on: May 19, 2021
Intermittent Antegrade Warm Blood Cardioplegia: What Is Next?
Antonio Maria Calafiore1, Piero Pelini1, Massimilliano Foschi2
1Department of Cardiac Surgery, Pope John Paul II Foundation, Campobasso Italy.
Abstract:
The introduction of warm heart surgery was a radical change in the concept of myocardial protection. In 1992, we applied a protocol for intermittent antegrade warm blood cardioplegia (CPL), which acquired some popularity for its simplicity and effectiveness. The possibility to deliver the warm blood CPL intermittently using the antegrade route attracted the attention of the scientific world, as the surgical procedure was less complicated. In this report, our aim is to focus on the changes that the protocol underwent over time and the reasons why these changes were made.
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