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Granulovirus GP37 Facilitated ODVs Cross Insect Peritrophic Membranes and Fuse with Epithelia

Xiangyang Liu1, Wei Fang2, Rui Fan3

  • 1College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China. liuxiangyang@henau.edu.cn.

Toxins
|March 7, 2019
PubMed

Insights

The Cydia pomonella granulovirus (CpGV) GP37 protein enhances nucleopolyhedrovirus (NPV) infectivity by damaging the insect midgut peritrophic membrane (PM) and promoting viral fusion with epithelial cells.

Area of Science:

  • Insect pathology
  • Molecular entomology
  • Virology

Background:

  • Cydia pomonella granulovirus (CpGV) GP37 exhibits synergistic effects on nucleopolyhedrovirus (NPV) infectivity.
  • The precise mechanism underlying this synergistic interaction remains largely unelucidated.

Purpose of the Study:

  • To investigate the mechanism by which CpGV GP37 enhances NPV infectivity in Spodoptera exigua larvae.
  • To elucidate the interaction of GP37 with the insect midgut and its role in viral entry.

Main Methods:

  • In vitro and in vivo binding assays to assess GP37 interaction with the peritrophic membrane (PM).
  • Quantitative PCR (qPCR) to evaluate the effect of GP37 on occlusion-derived virions (ODV) crossing the PM.
  • R18-labeling experiments to study the fusion of ODVs with insect midgut epithelia.

Main Results:

  • GP37 binds efficiently to the Spodoptera exigua larval midgut PM.
  • GP37 damages the PM's structure, increasing its permeability and facilitating ODV passage.
  • GP37 promotes the fusion of ODVs with insect midgut epithelial cells.

Conclusions:

  • The synergistic mechanism of GP37 involves PM damage, enhanced permeability, and promotion of ODV fusion with midgut epithelia.
  • These findings deepen the understanding of baculovirus synergistic factors.
  • Provides a basis for developing more effective baculoviral insecticides.
Keywords:
Cydia pomonella granulovirusGP37brush-border membrane vesicleperitrophic membranesynergistic mechanism

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