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Comparative transcriptome analysis of Eogammarus possjeticus at different hydrostatic pressure and temperature
Jiawei Chen1,2, Helu Liu1, Shanya Cai1,2
1Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, 572000, China.
Scientific Reports
|March 7, 2019
Summary
Marine amphipods show remarkable pressure tolerance, surviving up to 20 MPa across temperatures. This resilience is linked to specific gene expression changes, offering insights into deep-sea adaptation mechanisms.
Area of Science:
- Marine Biology
- Environmental Physiology
- Molecular Ecology
Background:
- Hydrostatic pressure significantly influences marine organism distribution.
- Shallow-water invertebrates exhibit surprising tolerance to high pressures.
- Molecular mechanisms of pressure acclimatization remain underexplored.
Purpose of the Study:
- To investigate the pressure tolerance of the shallow-water amphipod Eogammarus possjeticus.
- To explore the molecular responses to hydrostatic pressure at different temperatures.
- To identify genes and biological processes involved in deep-sea acclimatization.
Main Methods:
- Exposure of E. possjeticus to various temperatures (5-20°C) and hydrostatic pressures (0.1-30 MPa) for 16 hours.
- Assessment of survival rates under experimental conditions.
- Transcriptome analysis of six experimental groups to identify gene expression changes.
Main Results:
- E. possjeticus demonstrated 100% survival at 20 MPa across all tested temperatures.
- Transcriptome assembly yielded 138,304 unigenes.
- Differential gene expression analysis identified 94 up-regulated genes under high pressure.
Conclusions:
- Pressure tolerance in E. possjeticus is influenced by temperature.
- Up-regulated genes suggest involvement of energy metabolism, antioxidation, immunity, lipid metabolism, membrane processes, genetic information processing, and DNA repair.
- These findings provide insights into the molecular basis of adaptation to deep-sea environments.
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