Microdissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors

Mouse incisors are regenerative tissues, which grow continuously throughout life and are good model for the study of epithelial stem cells. The study of dental epithelial stem cells allows investigation of a variety of basic biological processes in the context of the stem cells. The ability to analyze dental epithelial stem cells in vitro has emerged as a powerful tool to understand how teeth are constructed and the signaling pathways that regulate ameloblast developmental processes. Here, we describe in detail our protocols for the culture of dental epithelial stem cells and the production of the cell lines. These techniques allow us to reproduce the differentiation process of ameloblasts and estimate the effect of specific genes ex vivo, as well as are a tool for studies on the mechanisms of normal and abnormal amelogenesis. They may also be applied to studies on other aspects of developmental biology and regenerative medicine using stem cells.