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Protocols to Study Dental Caries In Vitro: Microbial Caries Models.
Bennett T Amaechi1, Livia M A Tenuta2, Antonio P Ricomini Filho3
1Department of Comprehensive Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA. amaechi@uthscsa.edu.
Methods in Molecular Biology (Clifton, N.J.)
|March 7, 2019
Summary
Dental caries develop from sugar interacting with dental biofilm. This study details static and continuous flow biofilm models used to investigate sugar cariogenicity and anticaries agents.
Area of Science:
- Oral microbiology
- Dental research
- Biomaterials science
Background:
- Dental caries is a multifactorial disease initiated by dental biofilm metabolism of dietary sugars.
- Understanding dental biofilm's role is crucial for developing effective caries prevention and treatment strategies.
- Biofilm models are essential tools for studying cariogenicity and evaluating anticaries agents.
Purpose of the Study:
- To describe and compare "static" and "continuous flow" dental biofilm models.
- To highlight the advantages, limitations, and applications of each model.
- To provide a resource for researchers studying dental caries and anticaries interventions.
Main Methods:
- Detailed description of the "static" biofilm model setup and operation.
- Detailed description of the "continuous flow" biofilm model setup and operation.
- Comparative analysis of the two models based on key parameters relevant to caries research.
Main Results:
- Static models offer simplicity but limited physiological relevance.
- Continuous flow models provide a more dynamic and physiologically relevant environment for biofilm studies.
- Both models have distinct applications in assessing sugar cariogenicity and anticaries agent efficacy.
Conclusions:
- Static and continuous flow biofilm models are valuable but distinct tools in caries research.
- The choice of model depends on the specific research question regarding sugar cariogenicity or anticaries effects.
- Further refinement of biofilm models will enhance our understanding and management of dental caries.
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