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Related Experiment Video

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Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation
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Add-on plasmonic patch as a universal fluorescence enhancer.

Jingyi Luan1, Jeremiah J Morrissey2,3, Zheyu Wang1

  • 11Department of Mechanical Engineering and Materials Science, Institute of Materials Science and Engineering, Washington University in St. Louis, St Louis, MO 63130 USA.

Light, Science & Applications
|March 7, 2019
PubMed
Summary

A novel plasmonic patch enhances fluorescence signals up to 100-fold, improving detection of low-abundance biological species. This universally applicable technology boosts sensitivity in bioassays without altering existing methods.

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Area of Science:

  • Biomedical optics
  • Nanotechnology
  • Analytical chemistry

Background:

  • Fluorescence techniques are vital in biomedical optics for bioimaging and species detection.
  • Weak fluorescence signals limit the sensitivity for detecting low-abundance biological targets.
  • Existing methods require improvement to meet demands for higher sensitivity.

Purpose of the Study:

  • To develop a simple, universal method for significant fluorescence signal enhancement.
  • To introduce a "plasmonic patch" for boosting fluorescence in various bioassays.
  • To demonstrate the plasmonic patch's utility in improving assay sensitivity and detection limits.

Main Methods:

  • Fabrication of a flexible, conformal elastomeric film with adsorbed plasmonic nanostructures (plasmonic patch).
  • Transfer of the plasmonic patch onto different surfaces to enhance fluorescence.
  • Implementation of the plasmonic patch in fluorescence-based immunoassays (microtiter plates, microarrays).

Main Results:

  • Achieved up to 100-fold uniform fluorescence enhancement on various surfaces.
  • Demonstrated over 100-fold improvement in sensitivity and limit of detection for immunoassays.
  • Confirmed the plasmonic patch's effectiveness in both microtiter plate and microarray formats.

Conclusions:

  • The plasmonic patch offers a simple, cost-effective, and universally applicable solution for fluorescence enhancement.
  • This technology significantly improves sensitivity and detection limits in existing analytical methodologies.
  • The approach is disease, biomarker, and application agnostic, providing a fundamental enabling technology.