Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

1.3K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.3K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.5K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.5K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.1K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.1K
Endoscopic Studies II: Thoracocentesis01:26

Endoscopic Studies II: Thoracocentesis

1.4K
Thoracentesis(Thoracocentesis), commonly known as pleural tap, is a medical procedure where a 22 gauge needle is inserted into the pleural space, the area between the lung and chest wall. This procedure is commonly performed to diagnose or treat various respiratory disorders.
Description
Excess pleural fluid or air may accumulate in some respiratory disorders in the thoracic cavity. To treat pleural effusion, a physician conducts thoracentesis by carefully piercing the chest wall and entering...
1.4K
Endoscopic Procedures I: Esophagogastroduodenoscopy01:29

Endoscopic Procedures I: Esophagogastroduodenoscopy

1.1K
An Esophagogastroduodenoscopy (EGD) is a diagnostic procedure in which an endoscopist uses a flexible, lighted endoscope to visualize the upper gastrointestinal (GI) tract. The procedure includes visualizing the oropharynx, esophagus, stomach, and the first part of the small intestine, the duodenum.
During an EGD, the endoscope can be used to:
1.1K
Endoscopic Procedures II: Colonoscopy01:25

Endoscopic Procedures II: Colonoscopy

701
The colon, or large intestine, is the final segment of the digestive system. Its primary functions include absorbing water and vitamins produced by gut bacteria and transforming waste from liquid to solid to form stool. In adults, the large intestine is approximately 5 feet long and consists of four main sections:
701

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-Nanoparticle Dynamics in Opto-Thermal Tweezers: Resolving the Temporal Resolution of Depletion Force Trapping.

ACS nano·2026
Same author

Robust carotid artery perfusion protocol for turtle brain fixation enabling multiscale imaging from magnetic resonance to super-resolution microscopy.

Methods (San Diego, Calif.)·2026
Same author

Advanced Microscopy Techniques.

Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer·2026
Same author

A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo.

bioRxiv : the preprint server for biology·2026
Same author

Optical sectioning in wide-field two-photon microscopy using temporal focusing and random illumination.

Optics letters·2026
Same author

Publisher Correction: Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology.

Nature communications·2026

Related Experiment Video

Updated: Jan 28, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

4.8K

High-resolution multimodal flexible coherent Raman endoscope.

Alberto Lombardini1, Vasyl Mytskaniuk1, Siddharth Sivankutty1

  • 11Aix-Marseille Univ, CNRS, Centrale Marseille, Institut Fresnel, Marseille, France.

Light, Science & Applications
|March 7, 2019
PubMed
Summary

This study introduces a novel fiber-based coherent Raman endoscope for deep tissue imaging. This label-free, multimodal nonlinear endoscope achieves high-resolution imaging, paving the way for real-time diagnostics.

More Related Videos

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

279
A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy
07:40

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Published on: August 31, 2022

1.7K

Related Experiment Videos

Last Updated: Jan 28, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

4.8K
A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

279
A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy
07:40

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Published on: August 31, 2022

1.7K

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Endoscopy

Background:

  • Coherent Raman scattering microscopy offers label-free, chemically specific imaging but is limited to shallow depths in tissues.
  • Current limitations in fiber delivery and signal collection hinder deep-tissue coherent Raman endoscopy.
  • Advancing in vivo optical histology requires overcoming these barriers for deeper tissue interrogation.

Purpose of the Study:

  • To develop a flexible, compact, multimodal nonlinear endoscope for deep-tissue coherent Raman imaging.
  • To enable distortion-less excitation pulse delivery and background-free nonlinear signal collection via a novel fiber design.
  • To achieve sub-micrometer spatial resolution and high-contrast imaging for real-time histopathology.

Main Methods:

  • Utilized a resonantly scanned hollow-core Kagomé-lattice double-clad fiber for pulse delivery and signal collection.
  • Integrated a miniature objective lens with a silica microsphere lens for sub-micrometer resolution.
  • Demonstrated imaging using coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG).

Main Results:

  • Developed a 4.2 mm outer diameter, 71 mm rigid length coherent Raman endoscope.
  • Achieved distortion-less femtosecond pulse delivery and background-free signal collection through the same fiber.
  • Obtained high-resolution, high-contrast endoscopic images of biological tissues with a 320 µm field of view at 0.8 fps.

Conclusions:

  • The developed endoscope overcomes previous limitations in coherent Raman endoscopy.
  • This technology enables label-free, deep-tissue imaging for potential intraoperative diagnosis and surgery guidance.
  • Paves the way for advanced real-time histopathology using optical methods.