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Updated: Jan 28, 2026

Serial Block-Face Scanning Electron Microscopy SBF-SEM of Biological Tissue Samples
Published on: March 26, 2021
Biological imaging with scanning electrochemical microscopy
Felipe Conzuelo1, Albert Schulte2, Wolfgang Schuhmann1
1Analytical Chemistry-Center for Electrochemical Sciences (CES), Faculty for Chemistry and Biochemistry, Ruhr-Universität Bochum, Universitätsstr. 150, 44780 Bochum, Germany.
Abstract:
Scanning electrochemical microscopy (SECM) is a powerful and versatile technique for visualizing the local electrochemical activity of a surface as an ultramicroelectrode tip is moved towards or over a sample of interest using precise positioning systems. In comparison with other scanning probe techniques, SECM not only enables topographical surface mapping but also gathers chemical information with high spatial resolution. Considerable progress has been made in the analysis of biological samples, including living cells and immobilized biomacromolecules such as enzymes, antibodies and DNA fragments. Moreover, combinations of SECM with comple-mentary analytical tools broadened its applicability and facilitated multi-functional analysis with extended life science capabilities. The aim of this review is to present a brief topical overview on recent applications of biological SECM, with particular emphasis on important technical improvements of this surface imaging technique, recommended applications and future trends.
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