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Updated: Jan 28, 2026

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
Published on: September 29, 2015
Multi-well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen
1Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel. venkat.raghavan@weizmann.ac.il.
Abstract:
Collagen is the most widely used substratum in cell culture and biomaterials applications. In this chapter, we describe a simple procedure to isolate collagen, which can be employed to a wide range of tissue sources, and subsequently use it to study the collagen crosslinking and stabilization abilities of various compounds. The protocol is designed for a multi-well format assay and thus can be used for simultaneous assessment of multiple number of compounds and can be easily adapted to a high-throughput screening setup.
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