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Updated: Jan 28, 2026

Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB
Published on: August 6, 2011
Comparison of Two PCR Assays for Trichomonas vaginalis
Chang-Suk Noh1, Sang-Su Kim2,3, Sung-Yul Park4
1Department of Internal Medicine, Hallym University Hangang Sacred Heart Hospital, Seoul 07247, Korea.
Abstract:
PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.
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