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Characterization of Botrytis cinerea from Table Grapes in Chile Using RAPD-PCR.

J R Thompson1, B A Latorre1

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Summary
This summary is machine-generated.

Random amplified polymorphic DNA (RAPD) analysis revealed genetic variability in Botrytis cinerea, a common grape pathogen. While primers could distinguish B. cinerea from other fungi, they did not differentiate hosts or origins, suggesting potential host-pathogen relationships warranting further study.

Keywords:
Botrytis bunch rotappleblueberrygray moldtomato

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Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Mycology

Background:

  • Botrytis cinerea is a significant pathogen affecting various crops, including table grapes.
  • Understanding the genetic diversity of B. cinerea is crucial for developing effective disease management strategies.
  • Previous studies have explored the genetic makeup of B. cinerea, but host-specific variations require further investigation.

Purpose of the Study:

  • To analyze the genetic variability of Botrytis cinerea isolates from different hosts and geographical origins in Chile using Random Amplified Polymorphic DNA (RAPD) analysis.
  • To determine if specific RAPD profiles can differentiate B. cinerea based on host plant or geographical source.
  • To assess the potential for host-specific relationships within B. cinerea populations.

Main Methods:

  • RAPD analysis was conducted on 29 B. cinerea isolates from table grapes and other crops using 29 decamer primers.
  • Specific primers (OPA4 and OPA11) were used to differentiate B. cinerea from other co-existing epiphytic fungi.
  • Further RAPD analysis with 19 primers on 15 selected isolates was performed to group isolates based on genetic similarity.

Main Results:

  • No single RAPD primer could differentiate B. cinerea isolates by host or geographical origin.
  • Primers OPA4 and OPA11 successfully distinguished B. cinerea from other fungi and amplified specific DNA fragments (1.2 kb, 1.10 kb, 0.7 kb) even from single conidia.
  • Three distinct genetic groups of B. cinerea were identified, with isolates from table grapes clustering in Group I, apple/tomato in Group II, and blueberry isolates in Group I or III.
  • Similarity coefficients ranged from 0.326 to 0.891, indicating significant genetic variability.

Conclusions:

  • RAPD analysis demonstrated genetic variability among B. cinerea isolates, suggesting potential host-pathogen relationships.
  • Specific DNA fragments amplified by primers OPA4 and OPA11 can serve as reliable markers for identifying B. cinerea.
  • Further research is necessary to elucidate the pathological significance of the observed genetic variability and potential host specificity.