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First Report of Potato Spindle Tuber Viroid in Costa Rica.

R Badilla1, R Hammond2, C Rivera3

  • 1Centro de Investigación en Biología Celular y Molecular (CIBCM), Universidad de Costa Rica.

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|March 8, 2019
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Summary
This summary is machine-generated.

Potato spindle tuber viroid (PSTVd) was detected in Costa Rican potato crops exhibiting leaf roll and dwarfism symptoms. This marks the first identification of PSTVd in the region, impacting potato production.

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Area of Science:

  • Plant Pathology
  • Virology
  • Agricultural Science

Background:

  • Potato plants (Solanum tuberosum) in Costa Rica displayed symptoms including leaf roll, dwarfism, chlorosis, and necrosis.
  • Previous reports of potato leafroll virus (PLRV) in Costa Rica and known associations between PLRV and viroids prompted suspicion of potato spindle tuber viroid (PSTVd).

Purpose of the Study:

  • To investigate the presence of potato spindle tuber viroid (PSTVd) in symptomatic potato plants in Costa Rica.
  • To confirm PSTVd infection through biological indexing in tomato plants.

Main Methods:

  • Leaf samples were collected from 122 symptomatic and 6 healthy potato plants across 10 plots in Cartago.
  • Total nucleic acids (TNAs) were extracted and tested for PSTVd using digoxigenin-labeled DNA probe hybridization (dot blot).
  • Positive PSTVd samples were used to inoculate tomato plants (Lycopersicum esculentum) for symptom observation and confirmation.

Main Results:

  • Dot blot hybridization revealed PSTVd in 71 out of 122 symptomatic potato plants.
  • Inoculation of tomato plants with TNAs from infected potatoes resulted in characteristic viroid symptoms (dwarfism, leaf deformation) in 11 out of 12 cases.
  • Symptomatic tomato plants tested positive for PSTVd via dot blot hybridization.

Conclusions:

  • Potato spindle tuber viroid (PSTVd) is confirmed to be present in potato crops in Costa Rica.
  • This study represents the first report of PSTVd in Costa Rica, highlighting a significant threat to potato cultivation.
  • The findings necessitate further research into PSTVd epidemiology and management strategies in the region.