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Updated: Jan 28, 2026

Failure of Cleaning Verification in Pharmaceutical Industry Due to Uncleanliness of Stainless Steel Surface
Published on: August 11, 2017
Bacteriological Control of Food Equipment Surfaces by Cleaning Systems. I. Detergent Effects
1National Dairy Laboratory, Ruakura Agricultural Research Centre, Private Bag, Hamilton, New Zealand.
A cleaning simulator showed that alkaline detergent is crucial for controlling Streptococcus faecalis on stainless steel. Sanitizers offered some bacterial control, while rinses had minimal impact on bacterial numbers.
Area of Science:
- Food Safety and Hygiene
- Microbiology
- Surface Science
Background:
- Maintaining hygienic stainless steel surfaces is critical in food processing environments.
- Understanding the efficacy of different cleaning system components against bacterial contamination is essential.
Purpose of the Study:
- To evaluate the effectiveness of a cleaning simulator in assessing soil and bacterial load changes on stainless steel.
- To determine the impact of various cleaning system components (detergent, sanitizer, rinses) on controlling Streptococcus faecalis.
Main Methods:
- A cleaning simulator subjected stainless steel surfaces to 36 soiling and washing cycles.
- Four distinct cleaning systems were tested, varying in the inclusion of iodophor rinse, alkaline detergent, and water rinses.
- Milk inoculated with Streptococcus faecalis was used as the soil, with bacterial counts monitored throughout the cycles.
Main Results:
- The cleaning simulator effectively tracked changes in soil and bacterial numbers over time.
- Alkaline detergent was the most significant factor in reducing Streptococcus faecalis numbers.
- Sanitizer provided moderate bacterial control, whereas rinses demonstrated minimal effectiveness.
Conclusions:
- The efficacy of cleaning systems is primarily driven by the detergent's ability to remove soil and reduce bacteria.
- Bacterial numbers correlated with the amount of residual soil and the inhibitory effect of pre-wash solutions.
- Streptococcus faecalis did not proliferate during the 9.5-hour intercycle rest period under tested conditions.
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