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Circular RNA circ_0002138 is down-regulated and suppresses cell proliferation in colorectal cancer

Haoyu Ruan1, Xuan Deng1, Liu Dong1

  • 1Department of Clinical Laboratory, Huashan Hospital, Fudan University, 200040, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|March 8, 2019
PubMed

Insights

Circular RNAs (circRNAs) are down-regulated in colorectal cancer (CRC). This study shows circ_0002138 inhibits CRC cell proliferation and may serve as a diagnostic biomarker.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are endogenous noncoding RNAs with emerging roles in human health.
  • The involvement of circRNAs in colorectal cancer (CRC) tumorigenesis is not well understood.

Purpose of the Study:

  • To investigate the expression and function of circ_0002138 in colorectal cancer (CRC).
  • To evaluate circ_0002138 as a potential diagnostic biomarker for CRC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) to measure circ_0002138 expression in 35 paired CRC tissues.
  • Fisher's exact test to correlate expression with clinicopathological factors.
  • Receiver operating characteristic (ROC) curve analysis for diagnostic value assessment.
  • In vitro functional assays to assess effects on CRC cell proliferation.

Main Results:

  • circ_0002138 was significantly down-regulated in CRC tissues compared to adjacent normal tissues (P < 0.001).
  • circ_0002138 expression showed a significant correlation with patient age.
  • The area under the ROC curve was 0.7249, indicating diagnostic potential.
  • circ_0002138 significantly inhibited CRC cell proliferation in vitro.

Conclusions:

  • circ_0002138 is a potential tumor suppressor in colorectal cancer.
  • circ_0002138 may serve as a novel biomarker for CRC diagnosis.
  • circ_0002138 could be a potential therapeutic target for CRC treatment.
Keywords:
BiomarkerCRCCirc_0002138Proliferation

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