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Curcumin suppresses wilms' tumor metastasis by inhibiting RECK methylation
Wei Jia1, Fuming Deng1, Wen Fu1
1Department of Pediatric Urology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, Guangdong, China.
Abstract:
Wilms' tumor (WT) is the most common kidney tumor of children. The transformation suppressor gene RECK, which codes membrane-anchored glycoprotein, frequently downregulates multiple matrix metalloproteinases in tumors. And curcumin, which is a polyphenlic compound separated from turmeric, has antitumor effects on various cancers. However, the correlation of WT, RECK and curcumin is still unrevealed. In this study, we evaluated that the methylation degree of RECK was much higher in WT than in adjacent non-tumor tissues. And RECK methylation was closely associated with tumor metastasis in WT patients. After curcumin treatment, the level of RECK methylation was decreased significantly. And the expression of MMP2 and MMP9 was reduced consequently. Moreover, the proliferation, invasion and migration ability of WT cells were suppressed after curcumin treatment. Meanwhile, the apoptosis rate of WT cells was increased simultaneously. In nude mice model, curcumin restrained ability of tumorigenicity and promoted apoptosis of WT cells. Together, our results suggest that the RECK methylation can serve as a prognostic biomarker of WT. Moreover, curcumin could inhibit RECK methylation, thereby abates the expression of MMPs, and suppresses the tumor progression and metastasis of WT.
Insights
Curcumin treatment reduces RECK methylation and MMPs, inhibiting Wilms' tumor (WT) progression and metastasis. RECK methylation may serve as a prognostic biomarker for WT, with curcumin offering therapeutic potential.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Wilms' tumor (WT) is the most common pediatric kidney cancer.
- The RECK gene, a tumor suppressor, regulates matrix metalloproteinases (MMPs).
- Curcumin, a turmeric compound, exhibits anti-cancer properties.
Purpose of the Study:
- To investigate the correlation between WT, RECK methylation, and curcumin.
- To evaluate curcumin's effect on RECK methylation and WT progression.
Main Methods:
- Assessed RECK methylation levels in WT tissues versus adjacent non-tumor tissues.
- Treated WT cells and a nude mice model with curcumin.
- Measured RECK methylation, MMP expression, cell proliferation, invasion, migration, and apoptosis.
Main Results:
- RECK methylation was significantly higher in WT tissues and associated with metastasis.
- Curcumin treatment decreased RECK methylation and MMP2/MMP9 expression.
- Curcumin suppressed WT cell proliferation, invasion, and migration while increasing apoptosis.
- Curcumin inhibited tumorigenicity and promoted apoptosis in a nude mice model.
Conclusions:
- RECK methylation is a potential prognostic biomarker for WT.
- Curcumin inhibits WT progression by reducing RECK methylation and MMP expression.
- Curcumin demonstrates therapeutic potential for treating Wilms' tumor.