Related Experiment Video

Updated: Jan 28, 2026

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro
16:13

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Published on: June 13, 2011

20.4K

ROS/TRPA1/CGRP signaling mediates cortical spreading depression

Liwen Jiang1,2,3, Dongqing Ma1,2, Blair D Grubb3

  • 1Centre for Neuroscience, Xi'an Jiaotong-Liverpool University, Suzhou, China.

The Journal of Headache and Pain
|March 8, 2019
PubMed
Abstract

Insights

Reactive oxygen species (ROS) and TRPA1 channels influence migraine by affecting cortical spreading depression (CSD). Inhibiting ROS and TRPA1 may offer therapeutic benefits for migraine prevention.

Area of Science:

  • Neuroscience
  • Pharmacology

Background:

  • Migraine prophylaxis targets the transient receptor potential ankyrin A 1 (TRPA1) channel and calcitonin gene-related peptide (CGRP).
  • Understanding the interplay of reactive oxygen species (ROS), TRPA1, and CGRP in cortical spreading depression (CSD) is crucial for migraine research.

Purpose of the Study:

  • Investigate the role of TRPA1 in CSD in vivo.
  • Explore the mechanisms of ROS/TRPA1/CGRP signaling in regulating cortical susceptibility to CSD.

Main Methods:

  • TRPA1 expression was detected using immunohistochemistry.
  • CSD was induced and monitored in rats and mouse brain slices using electrophysiology and intrinsic optical imaging.
  • ROS levels were assessed via malondialdehyde (MDA) analysis.

Main Results:

  • TRPA1 deactivation reduced CSD susceptibility and ipsilateral MDA levels in rats.
  • In mouse slices, H2O2 facilitated CSD, an effect blocked by antioxidants and a TRPA1 antagonist.
  • CGRP blockade prolonged CSD latency, reversed by H2O2 and a TRPA1 agonist.

Conclusions:

  • ROS/TRPA1/CGRP signaling is critical in modulating cortical susceptibility to CSD.
  • Inhibiting ROS and deactivating TRPA1 channels show therapeutic potential for stress-triggered migraine via CGRP pathways.
Keywords:
Calcitonin gene-related peptideCortical spreading depressionMigraineReactive oxygen speciesTransient receptor potential ankyrin a 1

More Related Videos

ROS Live Cell Imaging During Neuronal Development09:25

ROS Live Cell Imaging During Neuronal Development

Published on: February 9, 2021

4.8K
Detection and Quantification of Calcitonin Gene-Related Peptide CGRP in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay07:14

Detection and Quantification of Calcitonin Gene-Related Peptide CGRP in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Published on: June 16, 2023

3.3K

Related Concept Videos

Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.4K
Long-term Depression01:05

Long-term Depression

Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
33.2K
Long-term Depression01:03

Long-term Depression

Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Calcium Ion Concentration Mechanism
If over...
3.3K
Freezing Point Depression and Boiling Point Elevation03:12

Freezing Point Depression and Boiling Point Elevation

Boiling Point Elevation
The boiling point of a liquid is the temperature at which its vapor pressure is equal to ambient atmospheric pressure. Since the vapor pressure of a solution is lowered due to the presence of nonvolatile solutes, it stands to reason that the solution’s boiling point will subsequently be increased. Vapor pressure increases with temperature, and so a solution will require a higher temperature than will pure solvent to achieve any given vapor pressure, including one...
39.9K
Depressants01:28

Depressants

Depressant drugs, including alcohol and sedative-hypnotics, diminish central nervous system activity by enhancing the action of gamma-aminobutyric acid (GABA), a neurotransmitter that reduces brain activity and promotes relaxation. These substances can have various therapeutic uses but also pose significant risks, especially when misused or combined.
Alcohol is a common depressant that can induce a sense of relaxation and reduced inhibition at low doses. Contrary to its occasional...
414
Paracrine Signaling01:21

Paracrine Signaling

Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
59.6K