Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

196.4K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.4K
What is Gene Expression?01:36

What is Gene Expression?

11.3K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.5K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.5K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.5K
5.5K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.8K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.8K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiecho Resting-State Functional Magnetic Resonance Imaging Analysis of Attention-Deficit/Hyperactivity Disorder Severity and Motor Dysfunction in a Pediatric Sample of Neurofibromatosis Type 1.

Pediatric neurology·2026
Same author

Selective Pyroptosis in NF1-Deficient Cells through PKCδ Agonism.

bioRxiv : the preprint server for biology·2026
Same author

MAPK-dependent release of GDNF from Schwann cells mediates tumor-independent pain in neurofibromatosis 1.

Science signaling·2026
Same author

A Randomized, Double-Blind, Pilot Study of N-Acetylcysteine for Motor and Cognitive Symptoms in Youth With Neurofibromatosis Type 1.

Pediatric neurology·2026
Same author

Valosin-Containing Protein Contributes to Plexiform Neurofibroma Formation and Represents a Novel Therapeutic Target.

Cells·2026
Same author

Decreased PP2A expression and activity represent a therapeutic target for plexiform neurofibroma.

Acta neuropathologica communications·2026

Related Experiment Video

Updated: Jan 28, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

12.2K

iGEAK: an interactive gene expression analysis kit for seamless workflow using the R/shiny platform.

Kwangmin Choi1, Nancy Ratner2

  • 1Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, 45229, USA. kwangmin.choi.phd@gmail.com.

BMC Genomics
|March 8, 2019
PubMed
Summary
This summary is machine-generated.

iGEAK is a user-friendly R/Shiny application for analyzing gene expression data from microarrays and RNA-seq. This tool simplifies differential gene expression analysis and downstream exploration for biologists without programming skills.

Keywords:
Differential expression (DE) analysisGenomicsGraphical user interfaceMicroarrayPipelineRRNA-seqShinyTranscriptomics

More Related Videos

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K
Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

10.9K

Related Experiment Videos

Last Updated: Jan 28, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

12.2K
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K
Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

10.9K

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Genomics

Background:

  • Microarray and RNA-seq technologies are essential for transcriptome analysis.
  • Existing analysis tools often require programming skills and lack user-friendly interfaces.
  • Bottlenecks in data processing hinder seamless analysis.

Purpose of the Study:

  • To develop an intuitive and interactive gene expression analysis tool.
  • To accelerate transcriptome analysis for biologists.
  • To provide a user-friendly alternative to complex bioinformatics software.

Main Methods:

  • Developed iGEAK, an R/Shiny-based client-side desktop application.
  • Created an interactive pipeline for gene expression data analysis.
  • Integrated sample selection, differential gene expression prediction, protein-protein interaction, and gene set enrichment analyses.

Main Results:

  • iGEAK provides a seamless and intuitive analysis pipeline for microarray and RNA-seq data.
  • Users can easily explore gene expression data and alter parameters for deeper biological insights.
  • The application facilitates differential gene expression prediction and downstream analyses.

Conclusions:

  • iGEAK empowers wet-bench biologists to analyze gene expression data without programming knowledge.
  • The tool offers essential analysis steps through a user-friendly graphical interface.
  • iGEAK aims to simplify the exploration and mining of transcriptome data.