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Related Experiment Videos

Cationized ferritin as a platelet-stimulating surface probe. Binding to platelets and effects on platelet function.

H Patscheke, R Dierichs

    European Journal of Cell Biology
    |March 1, 1986
    PubMed
    Summary

    Polycationic ferritin derivatives (CF) activate platelets, causing shape change, aggregation, and secretion. However, CF-induced activation and agglutination do not rely solely on neutralizing negative surface charges.

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    Neurology·2008

    Area of Science:

    • Biochemistry
    • Hematology
    • Molecular Biology

    Background:

    • Polycationic ferritin derivatives (CF) are potent platelet agonists.
    • These derivatives include those with primary (CFah) or tertiary (CFdmp) amino groups.
    • CF induces platelet shape change, aggregation, secretion, and agglutination, particularly with EDTA.

    Purpose of the Study:

    • To investigate the mechanisms of CF-induced platelet activation and agglutination.
    • To explore the role of platelet surface sites and their mobility in CF binding.
    • To determine the relationship between CF concentration, activation, and agglutination.

    Main Methods:

    • Platelet activation assays using CFah and CFdmp.
    • Inhibition studies with neuraminidase, PGE1, indomethacin, and creatine kinase/creatine phosphate.

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  • Analysis of CF binding to platelets at different temperatures and after fixation.
  • Kinetic analysis of CF binding and activation.

    • Main Results:

    • CF induced shape change, aggregation, secretion, and agglutination.
    • Neuraminidase, PGE1, indomethacin, or creatine kinase/creatine phosphate inhibited CF-induced activation.
    • Neuraminidase and PGE1 enhanced CF-induced agglutination, suggesting an inverse relationship.
    • Platelet binding capacity for CF depended on membrane mobility and was only slightly increased by activation.
    • A small subset of neuraminidase-sensitive sites is crucial for CF-induced platelet activation.

    Conclusions:

    • CF-induced platelet activation and agglutination are complex processes.
    • Platelet surface charge neutralization is not the sole mechanism.
    • Membrane mobility of binding sites plays a role in CF binding and activation.
    • Specific neuraminidase-sensitive sites are essential for CF-mediated platelet activation.