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Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED-FCS

Erdinc Sezgin1, Falk Schneider2, Silvia Galiani2

  • 1MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK. erdinc.sezgin@rdm.ox.ac.uk.

Nature Protocols
|March 8, 2019
PubMed
Summary

Super-resolution microscopy combined with fluorescence correlation spectroscopy (STED-FCS) allows direct observation of molecular diffusion in live cells. This technique bridges the gap between spatial and temporal resolution for studying cellular dynamics.

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