Related Experiment Video
Updated: Jan 28, 2026

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
Published on: October 29, 2013
A non-sacrificial method for the quantification of poly(ethylene glycol) grafting density on gold nanoparticles for
1State Key Laboratory of Supramolecular Structure and Materials , College of Chemistry , Jilin University , Changchun 130012 , P. R. China .
Abstract:
The grafting density of poly(ethylene glycol) (PEG) on nanoparticle (NP) surfaces is the most important parameter determining the interaction of nanoparticles with serum proteins, the subsequent sequestration of the nanoparticle from the bloodstream by the mononuclear phagocyte system, and the eventual delivery efficiency to tumor tissues. However, the majority of in vivo studies do not characterize or report the grafting density of PEG on nanoparticles due to a lack of feasible characterization methods, making it difficult to evaluate the published studies and reconcile apparent conflicting results. Herein, we develop a facile and non-sacrificial 1H NMR analytical approach for the quantitative characterization of grafting density of thiol-terminated PEG (HS-PEG) on gold NPs (GNPs). A multi-Lorentzian-splitting algorithm is used to distinguish the NMR signal of free PEG from those of the grafted ones, therefore allowing in situ monitoring of the grafting process to study the effects of GNP sizes, PEG molecular weights and NP capping ligands on grafting rates and grafting densities. The main advantage of this method is that it is not limited by the types of terminal functional groups on PEG, surface chemistry of the nanoparticles or their composition. It also provides a set of critical and standard guides for characterization of the PEG grafting density on nanoparticles for in vivo biological and biomedical studies.
Related Concept Videos
Applications of the Ideal Gas Law: Molar Mass, Density, and Volume
Density
Current Density
Bulk Density of Aggregate
Most natural mineral aggregates, like sand and gravel,...
Strain-Energy Density
In the elastic region of a material, the relationship between the stress and the strain is linear and follows Hooke's Law. The strain energy density in this region...
Density and Archimedes' Principle

